Through an interaction with a protein called the

"Cell signals have to be tightly controlled," says Dr. Richard G.W. Anderson, chairman of cell biology and senior author of the study. "If the signaling machines do not work, which can happen when the cell doesn't have enough cholesterol, the cell gets the wrong information, and disease results."

Though it has earned a bad reputation for its role in heart disease, the fact that cholesterol is essential for the health of cell membranes long has been understood. The cell membrane, which is fluid in nature, contains cholesterol.

Keeps Cancer-Related ERK Under Control

Dr. Anderson's research focuses on regions of the membrane where cholesterol is enriched. These regions, called "lipid domains," are more rigid than the rest of the cell membrane because of cholesterol and play a critical role in organizing signaling machinery at the cell surface. The correct arrangement of signaling modules in these domains is vital for communication inside the cell and is dependent on proper levels of cholesterol.

While studying how cholesterol moves to the membrane to get to lipid domains, Dr. Anderson, who holds the Cecil H. Green Distinguished Chair in Cellular and Molecular Biology, and colleagues found that cholesterol can work outside the membrane to regulate a key signaling pathway that occurs inside the cell.

Through an interaction with a protein called the "oxysterol binding protein" (OSBP), cholesterol holds together a group of enzymes that deactivates extracellular signal-related kinase (ERK). Overactive ERK is associated with multiple cancers.

When the amount of cholesterol in lipid domains is normal, the OSBP-cholesterol complex keeps the amount of active ERK under control. When cholesterol in the domains gets too low, however, the complex falls apart, leading to abnormally high levels of active ERK.

Dr. Anderson and colleagues noticed that OSBP has binding sites for both cholesterol and the other proteins in the complex. They believe that when cholesterol binds OSBP it changes shape to bind the key enzymes in a way that allows them to work together to control deactivation of ERK. When lipid domain cholesterol gets low, OSBP loses its cholesterol and no longer is able to bind the enzymes that deactivate ERK, keeping it active.

May Have Application to Other Diseases

"OSBP appears to work like a cholesterol-regulated scaffolding protein that controls a key signaling pathway," Dr. Anderson explains.

"This work shows a new way that lipids can regulate key signaling pathways and raises the possibility that other lipid-regulated signaling scaffolds can malfunction in other diseases," he adds.

Other UT Southwestern contributors to the study were Dr. Jian Weng, assistant professor of cell biology, and Dr. Ping-Yuan Wang, postdoctoral researcher in cell biology and lead author.